http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112940118-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-4742 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-92 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-56 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-57484 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-574 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-4741 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-574 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 |
filingDate | 2021-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-112940118-B |
titleOfInvention | Monoclonal antibody of anti-CK 8 protein, cell strain, preparation method and application thereof |
abstract | The invention relates to a monoclonal antibody capable of identifying a human leukocyte differentiation antigen CK8, a secretory cell strain, a preparation method thereof and application thereof in immunodetection. The technical scheme includes that an amino acid sequence from 340 to 365 at the C terminal of CK8 protein is selected as an antigen peptide, and the antigen peptide is coupled with KLH carrier protein to obtain the immunogen; the amino acid sequence of the antigen peptide is shown as SEQ ID1, the mouse is immunized, and the mouse hybridoma cell strain 14C2 capable of efficiently secreting the anti-CK 8 protein monoclonal antibody and the anti-CK 8 protein monoclonal antibody secreted by the cell strain are obtained through cell fusion, screening and subcloning. The antibody obtained by the scheme has high specificity and sensitivity, can specifically recognize cells expressing CK8 protein, and is suitable for immunological detection, particularly immunohistochemical detection. |
priorityDate | 2021-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 244.