| abstract |
The invention discloses a trimethoprim hapten TMPH, an artificial antigen, an antibody, a preparation method and application thereof. The invention firstly provides a trimethoprim hapten TMPH, and further takes an artificial antigen obtained by coupling the trimethoprim hapten TMPH and carrier protein as an immunogen to immunize a mouse to prepare the trimethoprim hapten TMPHTo trimethoprim monoclonal antibodies. The antibody prepared by the invention has better titer, specificity and affinity, and the minimum detection limit on trimethoprim is 0.025ng/mL and IC 50 The kit is 0.80ng/mL, has a linear range of 0.12-5.19 ng/mL, has the characteristics of simplicity, rapidness, strong specificity, wide linear range and high sensitivity, and has a good application prospect and a wide development space in the rapid and effective detection of trimethoprim. |