http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112931198-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-008 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N13-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 |
| filingDate | 2019-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112931198-B |
| titleOfInvention | A kind of preparation method of pineapple cold-resistant germplasm |
| abstract | The invention relates to a method for preparing pineapple cold-resistant germplasm, and belongs to the technical field of pineapple cold-resistant germplasm preparation. The preparation method of the pineapple cold-resistant germplasm in the present invention first uses EMS to carry out mutagenesis of the pineapple embryogenic suspension cells; then the embryogenic suspension cells after the mutagenesis are aseptically cultured on the regeneration medium for 30-50 days; then Adjust the temperature to 3-7°C for 4-6 days to obtain surviving mature embryos; finally, carry out germination and rooting culture on the surviving mature embryos to obtain proliferative sprouts; then adjust the temperature to 3-7°C for sterile Cultivate the proliferating sprouts for 4-6 days; select the surviving proliferating sprouts, which are pineapple cold-resistant germplasm. In the method of the present invention, the key link can be completed in the laboratory, and the experimental conditions are controllable; a large number of pineapple individuals can be screened for cold resistance in a short period of time, and the cold-resistant germplasm can be obtained through rapid screening, which can obviously accelerate the resistance of pineapple. The progress of cold breeding. |
| priorityDate | 2019-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 70.