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filingDate 2019-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c215a6bc50c95b3df2e3112b5e1f27e
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publicationDate 2021-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-112931197-A
titleOfInvention A kind of preparation method of pineapple tissue culture seedling
abstract The invention relates to a preparation method of pineapple tissue culture seedlings, belonging to the technical field of pineapple tissue culture seedling preparation. In the method for preparing pineapple tissue culture seedlings in the present invention, buds on pineapple plants are taken to induce embryogenic callus, then an embryogenic suspension cell line is established, and the pineapple tissue culture seedling is obtained by regenerating the embryogenic suspension cell line. The method of the present invention has a large reproduction coefficient, and a bud can obtain tens of thousands of independent cells through callus and suspension cell lines, and these independent cells can develop into a single plant under suitable conditions; on the other hand Suspension cells are easy to preserve. When a certain variety of seedlings are needed, the preserved suspension cell line can be used to quickly regenerate corresponding seedlings, which shortens the cultivation time of pineapple seedlings and effectively improves the viability of pineapple seedlings. Continuous supply.
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