| abstract |
The present application relates to the technical field of immunological detection, and specifically discloses an immunological detection method for imatinib and/or N-desmethyl imatinib. The magnetic particle working solution of the conjugate and the enzymatic labeling working solution containing the conjugate formed by coupling the hapten and the marker were mixed evenly, and after the incubation reaction and washing steps were performed in sequence, the indicator solution was added to show the result. color and collect the light signal; the structure of the hapten is shown in Equation 10: Antibodies are produced in response to antigens, which are conjugates formed by coupling a hapten and a carrier protein; the indicator solution contains an indicator for quantitative detection of the concentration of the label. The present application can simultaneously measure imatinib and N-desmethyl imatinib, and has the advantages of strong specificity, sensitivity, simple operation, time-saving and labor-saving. |