http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112921043-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0061 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y110-03002 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1031 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-84 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 |
| filingDate | 2021-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112921043-B |
| titleOfInvention | Mutant nucleic acid, expression vector, laccase mutant with high specific activity and preparation method thereof |
| abstract | The invention discloses a mutant nucleic acid, an expression vector, a method for preparing a laccase mutant with high specific activity and a preparation method thereof, wherein the sequence of the mutant nucleic acid is shown as SEQ ID NO: 1 is shown in the specification; the invention rapidly obtains the mutant laccase gene by ep-PCR technology to form a huge mutant library; meanwhile, a histidine tag is constructed at the N-terminal of the protein, so that the subsequent high-throughput screening of mutant strains by using the tag is facilitated, the heterologous expression and the transformation of laccase genes in pichia pastoris are realized, the specific activity of the recombinant pichia pastoris mutant strain enzyme protein is improved by about 2 times, the use cost of the laccase is reduced, and the industrial application potential of the recombinant pichia pastoris is improved. |
| priorityDate | 2021-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 36.