http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112921020-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y402-02011 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2021-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112921020-B |
| titleOfInvention | Algin lyase mutant for relieving divalent metal ion dependence and application thereof |
| abstract | The invention relates to the technical field of protein engineering, in particular to an alginate lyase mutant for relieving the dependence of divalent metal ions and application thereof. The mutant is alginate lyase amino acid sequence which takes polyG as a specific substrate when carrying out enzymolysis in PL7 family alginate lyase, at least in the same three-dimensional structure position with aspartic acid D146 in AlgAT5, or the amino acid at D146 position is mutated into amino acid with positive charge, glycine, alanine, valine, leucine or isoleucine based on the multi-sequence alignment of the structure. The beneficial mutations can provide theoretical and technical support for the industrial development and application of alginate lyase and promote the green and efficient preparation of alginate oligosaccharides. |
| priorityDate | 2021-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 347.