| abstract |
The invention belongs to the technical field of GnRH detection kits, and in particular relates to an ELISA kit for detecting GnRH antibody titers in animal serum. The coated antigen also includes a coating solution and a blocking solution; the amino acid sequence of the GnRH polypeptide is shown in the sequence table SEQ ID NO.1; the GnRH kit also includes standard negative serum, standard positive serum, horseradish peroxidation Enzyme HRP-labeled goat anti-cat IgG, washing solution, diluent, substrate solution and stop solution; GnRH polypeptide antigen was used as coating antigen, and ELISA detection method was used to detect GnRH antibody titer, so as to understand the reproduction of animals at this stage It can save the time of coating and blocking, shorten the detection process of GnRH, and can stably detect the GnRH antibody titer in animal serum. |