http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112899349-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_610a491d3badd24d1ea60fda8d02b7e3 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 |
| filingDate | 2020-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1e9d6e32223440b145db8dc46c985beb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_026cde4da2180668c9af2de4148a1d0c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ad3afff3725317d9965c5ca59cfb1c03 |
| publicationDate | 2021-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112899349-A |
| titleOfInvention | Visual nucleic acid detection method for simultaneous detection of one or more target nucleic acids and application thereof |
| abstract | The present invention provides a visual nucleic acid detection method for simultaneously detecting one or more target nucleic acids, the method comprising the following steps: 1) preparing a reaction mixture, wherein the reaction mixture comprises one or more template DNA, DNA polymerase , DNA nickase 1, DNA nickase 2 and one or more probes; 2) The reaction mixture obtained in step 1) is placed at a constant temperature to perform a constant temperature exponential amplification reaction; 3) Colloidal gold detection is prepared Buffer; 4) Mix the reaction product of step 2) with the buffer of step 3), put it into a colloidal gold test strip for detection, and interpret the result according to the detection strip. Compared with the prior art, the present invention has the following advantages: the method of the present invention adopts the visual reading of test paper items, is simple to operate, requires low professional skills, does not require special technical training, and greatly expands its application range; and Significantly reduces inspection costs. |
| priorityDate | 2020-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 61.