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filingDate 2021-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_796eda4966e8f3b5b6419927f1a79313
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publicationDate 2021-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-112899347-A
titleOfInvention PH buffer-free inactivated virus preservation solution compatible with various magnetic bead method virus nucleic acid extraction kits
abstract The invention relates to the technical field of molecular biology, in particular to a virus preservation solution of a high-compatibility paramagnetic particle method virus nucleic acid extraction kit without a PH buffer solution, which comprises the following components: guanidine hydrochloride, lithium dodecyl sulfate, ammonium sulfate, Triton X-100 and other protein denaturants and nucleic acid protecting components can inactivate viruses, DNA and RNA enzymes quickly, and have the function of cracking virus samples and the function of protecting the stability of DNA and RNA simultaneously. The virus preservation solution does not need to add PH buffer solution such as tris (hydroxymethyl) aminomethane hydrochloride, ethylene diamine tetraacetic acid and the like, isopropanol or ethanol and the like which possibly cause components, does not interfere with subsequent extraction, can be compatible with various magnetic bead method virus nucleic acid extraction kits commonly used in the market, and when extracting nucleic acid, a sample and lysis solution can be mixed according to any proportion between the proportion suggested by the kit and 100 percent, namely, the lysis solution can be added little or not added, so that the use amount of the sample is increased, and more virus nucleic acid and more accurate detection results are obtained.
priorityDate 2021-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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