http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112889813-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-0284 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-0221 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-0226 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N1-02 |
| filingDate | 2021-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112889813-B |
| titleOfInvention | A cryopreservation method for human umbilical cord mesenchymal stem cells with high cell viability |
| abstract | The invention relates to the technical field of stem cells, in particular to a cryopreservation method for human umbilical cord mesenchymal stem cells with high cell survival rate. A method for cryopreserving human umbilical cord mesenchymal stem cells with high cell viability, the steps comprising: (1) digestion and termination of digestion: enzymatic digestion and digestion of human umbilical cord mesenchymal stem cells of no more than 5 generations for 1 to 5 minutes, and then adding Stop the digestion with stop solution; (2) Washing and centrifugation: Discard the supernatant by centrifugation, add detergent and repeat the washing and centrifugation 2 to 3 times; (3) Program freezing: add pre-cooled cells at a cell density of 1×10 7 /ml Cryopreservation solution, after resuspension, draw 1-5 m of cell suspension into a programmed cooling device for programmed cooling and cryopreservation. In the present invention, the survival rate of cells before and after cryopreservation is not significantly reduced, the survival rate is above 97%, and the number of cells is still relatively high by cooperating with a suitable cryopreservation step in the presence of a special cryopreservation solution. concentration. |
| priorityDate | 2021-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 39.