http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112881676-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2e144be1ccb92ecc552ebd77a80a4faf |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-533 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-533 |
filingDate | 2021-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e9472acc4fee98127ab75feea155543 |
publicationDate | 2021-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-112881676-A |
titleOfInvention | Method for manufacturing time-resolved fluorescence immunoassay quantitative chromatography test paper strip for plasticizer in food |
abstract | The invention discloses a method for manufacturing a time-resolved fluorescence immunoassay quantitative chromatography test strip for plasticizers in food, which comprises the following steps: on being fixed in the base plate with the reaction membrane, again with sample absorption pad and water absorption pad overlap joint in the both ends of reaction membrane respectively, the interval is equipped with appearance district and microballon district on the sample absorption pad, is equipped with detection line and quality control line on the reaction membrane, including following step: step one, preparing a time-resolved fluorescent microsphere solution with a plasticizer-resistant monoclonal antibody coated on the surface; step two, preparing a plasticizer-bovine serum albumin conjugate solution and a goat anti-mouse IgG secondary antibody solution; step three, spraying the time-resolved fluorescent microsphere solution to a microsphere area, and performing negative pressure treatment; step four, respectively spraying the plasticizer-resistant monoclonal antibody solution and the goat anti-mouse IgG secondary antibody solution onto the detection line and the quality control line; step five, ultrasonic treatment; and sixthly, drying in an oven. The method has the beneficial effect of quickly and accurately detecting the plasticizer content. |
priorityDate | 2021-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 30.