| abstract |
The invention relates to the technical field of bioengineering, in particular to five standard strains of Bacillus cereus with independent intellectual property rights in my country and conforming to domestic transmission laws, which can be used as standard reference strains in different fields such as food, medicine and clinical testing. The deposit numbers are: GDMCC 60865, GDMCC 60866, GDMCC 60867, GDMCC 60868 and GDMCC 60869. The five standard strains have typical physiological and biochemical characteristics, and the information on sample origin, genetic background, drug resistance, and virulence genes is clear. Fully meet the requirements for standard strains in food, medicine, and clinical testing. The present invention also relates to a set of specific target genes and corresponding PCR primers for detecting and identifying the above-mentioned five standard strains. Finally, the present invention also provides a lyophilized protective agent for Bacillus cereus with high survival rate and specificity, which can be used for long-term storage of the standard strain of the present invention. |