http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112877287-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-998 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0663 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-145 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0654 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-06 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-077 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06 |
filingDate | 2020-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-112877287-B |
titleOfInvention | Application of bullacta exarata polypeptide in preparation of osteogenic differentiation accelerant for mesenchymal stem cells |
abstract | The invention belongs to the technical field of stem cells, and particularly relates to application of mud snail polypeptide in preparation of a bone marrow mesenchymal stem cell osteogenic differentiation accelerant. The invention prepares a mud snail polypeptide by the following preparation method: cleaning the mud snail, taking a soft tissue, freeze-drying, crushing, and adding 3 times of water by weight to obtain mud snail homogenate; adding papain with the weight 0.02 times that of the mud snail homogenate, adjusting the pH to 6, and carrying out thermostatic water bath at 55 ℃ for 4 hours to obtain mud snail enzymolysis liquid; heating in water bath at 95 deg.C for 20 min, centrifuging at 5000r/min, and collecting supernatant to obtain crude bullacta exarata extract; the crude mud snail extracting solution passes through a nanofiltration membrane with the aperture of 2nm to obtain a fine mud snail extracting solution; and (3) placing the extracting solution of the mud snail into a vacuum freeze dryer for vacuum freeze drying to obtain the mud snail polypeptide. Experiments show that the mud snail polypeptide prepared by the invention can effectively promote osteogenic differentiation of bone marrow mesenchymal stem cells. |
priorityDate | 2020-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 38.