| abstract |
Methods and systems are provided for increasing the phosphate solubilizing activity of microorganisms. Microorganisms identified by using the disclosed methods and systems are also provided. The method comprises the steps of: identifying a coding sequence associated with phosphate solubilization, altering the coding sequence associated with phosphate solubilization, functionally linking the altered coding sequence associated with phosphate solubilization to a promoter and introducing the altered phosphate solubilization-related coding sequence under the control of the promoter into the microorganism, and the system includes instructions for the steps. Alterations to the coding sequence may include, for example, codon randomization of native codons, deletion of coding sequences, and insertion of regulatory sequences. |