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filingDate 2021-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2021-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-112858677-A
titleOfInvention Method, reagent strip and kit for labeling pLDH (layered double hydroxide) monoclonal antibody against plasmodium vivax)
abstract The invention relates to a method for marking pLDH monoclonal antibody of plasmodium vivax, which comprises the following steps: (1) preparing a colloidal gold solution; (2) adjusting the pH value of the prepared colloidal gold solution to be 1.0-1.5 lower than the optimal pH value; (3) the labeled anti-plasmodium vivax pLDH monoclonal antibody is added at one time, and the adding amount is 60 to 70 percent lower than the amount of the most suitable labeled anti-plasmodium vivax pLDH monoclonal antibody; (4) adding a sealing agent and a stabilizing agent, and marking; breaking the safety range, placing the immune gold under a relatively unstable and controllable condition to obviously improve the color development intensity and the sensitivity of the product, wherein the relatively unstable and controllable condition refers to that the pH value of the colloidal gold label is slightly lower than the isoelectric point of the to-be-labeled anti-plasmodium vivax pLDH monoclonal antibody, the dosage of the labeled anti-plasmodium vivax pLDH monoclonal antibody is unsaturated, and the sealant and the stabilizer are added simultaneously, so that the color development intensity of the final antibody on the product is obviously deepened, and the sensitivity is obviously improved.
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