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publicationDate 2021-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-112858531-A
titleOfInvention A kind of Eucommia ulmoides leaf and its extract HPLC characteristic chromatogram establishment method
abstract The invention discloses a method for establishing an HPLC characteristic spectrum of Eucommia ulmoides leaves and its extract and its fingerprint spectrum. The method includes: step 1 preparation of a test solution; step 2 preparation of a reference solution; step 3 chromatographic conditions: using ten Octaalkylsilane - bonded silica gel is used as filler; mobile phase A is acetonitrile, mobile phase B is 0.1% phosphoric acid solution, and gradient elution is performed; detection wavelength: 206nm; column temperature: 10‑30℃; flow rate: 1.0ml.min‑ 1 ; Injection volume: 10 μl; Step 4: Measure to obtain the fingerprint of the purple light sheet. The present invention also conducts methodological verification on the establishment method of the fingerprint. The characteristic spectrum of Eucommia leaf and its extract determined by the invention has high precision, good stability and good repeatability, can effectively control the quality of Eucommia leaf and its extract, and ensure the quality of Eucommia leaf, extract and related products. Uniform, stable, safe and effective.
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