http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112858243-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6432 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2021-02-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112858243-B |
| titleOfInvention | Method for visually monitoring cytochrome c in living cell and evaluating vomitoxin cytotoxicity |
| abstract | The invention discloses a method for visually monitoring cytochrome c in living cells and evaluating vomitoxin cytotoxicity, and belongs to the technical field of fluorescence imaging and biosensing. The method of the invention utilizes the unmarked fluorescent material CdTe @ MIP which can specifically identify Cyt c as a probe, combines the high selectivity of MIP identification and the high sensitivity and yellow luminescence performance of fluorescence detection, and realizes the fluorescence imaging of Cyt c in cells. In the method, when the HepG2 cell is induced to die by DON, Cyt c generated by mitochondria is released to cytoplasm, and visual real-time monitoring of Cyt c in living cells and visual evaluation of DON-induced apoptosis level are realized by using the imaging result of a laser confocal microscope, thereby enriching the evaluation means of DON toxicity and providing an early warning means for food safety. |
| priorityDate | 2021-02-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 65.