http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112852848-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2039-55561 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-39 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-35 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-39 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 |
| filingDate | 2021-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112852848-B |
| titleOfInvention | Mycobacterium tuberculosis fusion protein AH vaccine containing codon optimization |
| abstract | The invention provides a codon-optimized mycobacterium tuberculosis fusion gene, a corresponding fusion protein and a vaccine, and application and a preparation method thereof in preventing tuberculosis. The antigen Ag85A secreted in the early stage of the infection of the mycobacterium tuberculosis and the latent expression antigen HspX are subjected to fusion expression, and the two antigen genes are expressed by flexible linker (GGGGS) 3 Ligation was performed, the fusion gene sequence was codon-optimized according to the codon bias of E.coli DNA, and then the gene sequence was ligated into the vector pET-30a (+) for E.coli expression. The purified recombinant protein Ag85A-HspX (AH for short) and polyinosi (PolyI: C) are used for intranasal immunization of mice, and the mice are proved to have good immunogenicity and protection against infection of the mycobacterium bovis. |
| priorityDate | 2021-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 127.