abstract |
The invention provides an NK trophoblast cell and a preparation method and application thereof, wherein the NK trophoblast cell expresses membrane proteins CD19, CD86, IL21, CD64 and CD 137L; the IL-21 is expressed on the surface of the NK trophoblast cell by forming an IL-21-CD8 alpha fusion protein with a CD8 alpha transmembrane region; the CD86 comprises an amino acid sequence shown as SEQ ID NO. 1; the IL-21-CD8 alpha comprises an amino acid sequence shown in SEQ ID NO. 2. The NK trophoblast cells constructed by the invention have high expression rate on CD19, CD86, IL21, CD64 and CD137L, and all factors synergistically activate the proliferation capacity of NK cells, thereby realizing the effect of inducing NK cells to differentiate and mature in vitro. |