http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112852695-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y401-01014 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2020-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112852695-B |
| titleOfInvention | Recombinant escherichia coli for producing isobutylamine as well as construction method and application thereof |
| abstract | The invention discloses a recombinant escherichia coli for producing isobutylamine and a construction method and application thereof. The inventor of the invention uses Escherichia coli Sval031 as a starting bacterium, integrates a gene coding L-valine decarboxylase derived from Streptomyces viridifaciens into the starting bacterium, and improves the expression strength of the valine decarboxylase by using an M1-93 promoter through a technology for regulating the expression strength of the gene on a chromosome, so that an isobutylamine high-producing strain ISO-001 is obtained, the yield of isobutylamine can reach 52.7g/L, and the conversion rate can reach 0.92 mol/mol. In order to further improve the production efficiency of isobutylamine, the inventor of the invention uses RBS library regulation and control technology to optimize the expression strength of VlmD gene, obtains a strain ISO-I with higher production efficiency of isobutylamine, and further improves the yield of isobutylamine by 66.4 g/L. The invention realizes the industrial fermentation production of isobutylamine and has very strong industrial application potential. |
| priorityDate | 2020-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 356.