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filingDate 2021-02-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2022-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2022-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-112852420-B
titleOfInvention Carbon quantum dot fluorescent probe and method for detecting thiamphenicol content
abstract The invention provides a carbon quantum dot fluorescent probe and a method for detecting thiamphenicol content in food and living cells. The preparation method of the carbon quantum dot fluorescent probe comprises the following steps: (1) crushing crayfish shells; (2) dispersing crayfish shell powder in ultrapure water, adding L-cysteine, transferring the mixture into a reaction kettle, and carbonizing at 200 ℃ for 8 hours by adopting a hydrothermal method; (3) and after cooling to room temperature, centrifuging the obtained product, filtering and collecting supernatant, filtering with a 0.22-micron filter membrane, dialyzing and purifying, freeze-drying the obtained solution to obtain carbon quantum dots, and dissolving the carbon quantum dots in water to obtain the carbon quantum dot fluorescent probe. The carbon quantum dot fluorescent probe provided by the invention can effectively quench by a static quenching mode, has the advantages of wide linear detection range, reasonable detection limit, high sensitivity and good selectivity, can be well applied to the rapid detection of trace thiamphenicol residues in foods and living cells, and is not interfered by complex environment.
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