http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112851776-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02E50-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-395 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-865 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-395 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 |
| filingDate | 2020-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112851776-B |
| titleOfInvention | A gene site-directed mutagenesis method and its application in stress resistance breeding |
| abstract | The invention discloses a gene site-directed mutation method and its application in stress resistance breeding. The method of the invention applies the cytidine deaminase base editing technology for the first time to mutate the global transcription regulator Spt15 on the genome of Saccharomyces cerevisiae, expands the mutation range of the transcription factor Spt15, realizes the precise mutation of the transcription factor Spt15, and obtains 36 mutant strains 14 strains, 18 strains and 19 strains with significantly improved tolerance were successfully and efficiently screened under the conditions of high glucose hypertonicity, high temperature and ethanol stress, of which 14 strains were resistant under these three stresses strains. The invention can solve the defect of insufficient stress tolerance of yeast cells in actual industrial fermentation. The method of the invention is simple and convenient to operate, and has high breeding efficiency, which lays a good foundation for future research on tolerance mechanism and molecular transformation and application of strains. |
| priorityDate | 2020-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 350.