abstract |
The invention discloses a construction method of a small intestine enteritis model of laying hens, which comprises the following steps: selecting 40 Roman powder laying hens in the egg laying peak period, and treating the Roman powder laying hens respectively as a control group and 4 toxin-counteracting treatment groups; the challenge treatment group was coccidial vaccine + different gradient clostridium perfringens type a treatment group, 9: 00 the coccidian vaccine is administered first, and the clostridium perfringens bacterial liquid is administered after half an hour; sampling 48 hours after the last drenching; the test period is 7 days, the feed is corn-soybean meal type basic feed, and the feeding management is carried out according to the conventional feeding management. The invention can explore the specific mechanism of the laying hen production performance reduction caused by factors such as nutrition, feed factors, stress and the like. |