| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ed45040c25720954487cd2e14c16052a |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6895
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-645 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6895
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 |
| filingDate |
2021-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4c3c6ffd42a9ba9e29d7c14dfd611496
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1952adf29bb38e67a2caa21851cdd52f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4031ce3856a6d9006d0549f27fd694bf
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d58fcbcefb441b8faff6507690bed59
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8b6c6730c145e60230533291be97f27e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9407f3da84737c14c97b15fcea97be89
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0167fea55d86a2ae456fd579406129bc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0bdca5ff27ff199c2033d34ab1d017d4 |
| publicationDate |
2021-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-112831591-A |
| titleOfInvention |
Identification method of SSR marker fingerprint of flammulina velutipes J3673 strain, construction method and application thereof |
| abstract |
The invention discloses an identification method of an SSR marker fingerprint of a flammulina velutipes J3673 strain, a construction method and application thereof, wherein the fingerprint consists of 6 pairs of SSR markers. The construction method comprises the following steps: (1) culturing hyphae; (2) extracting genome DNA; (3) detecting SSR molecular markers; (4) and (5) detecting by capillary electrophoresis. The application comprises the following steps: and performing SSR marker amplification on the flammulina velutipes strains, comparing the obtained banding patterns with the fingerprint spectrum, and obtaining the flammulina velutipes J3673 strains if the banding patterns are consistent with the fingerprint spectrum. Compared with conventional morphological detection, antagonism test and fruiting test, the method has the advantages of short detection time, high accuracy and good repeatability, and has the specificity of the flammulina velutipes J3673 strain in the collected 105 flammulina velutipes cultivation main culture strains at home and abroad. |
| priorityDate |
2021-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |