http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112831588-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6895 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6895 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 |
| filingDate | 2021-02-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112831588-B |
| titleOfInvention | Molecular marker and application thereof, and method for identifying germplasm of sapindus mukorossi and sapindus chuannanensis |
| abstract | The invention discloses a molecular marker and application thereof and a method for identifying the germplasm of sapindus mukorossi and sapindus chuannanensis, wherein the molecular marker comprises primers Samuk07G0006800, Samuk02G0156900 and/or Samuk01G0267400 and is used for identifying the germplasm of sapindus mukorossi and sapindus chuannanensis; extracting DNA of a sapindus mukorossi or sapindus chuannanensis tender leaf sample to be detected; performing PCR amplification according to the SSR molecular marker; detecting on a capillary electrophoresis machine; analyzing the original data of the sample to construct a phylogenetic tree; and determining the germ plasm amplification product as soapberry or Sichuan soapberry. The fluorescence labeling SSR capillary electrophoresis detection method established based on the gene sequencer is effective combination of a fluorescence sequencing technology and an SSR molecular labeling technology, realizes high-efficiency and accurate acquisition of the length of an SSR molecular labeling amplification product, can distinguish difference fragments within 2bp, accurately acquires the length of the amplification product, and has more stable, accurate and high-efficiency detection results. |
| priorityDate | 2021-02-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 26.