http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112831499-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d9de52bba13e16028dffa8775e3f3f28 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-127 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P35-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K31-7088 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P35-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 |
| filingDate | 2021-02-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cf402f71a447b720a726f3bbbbc1e864 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d39e351a3574fe1490bda8fec51d666f |
| publicationDate | 2021-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112831499-A |
| titleOfInvention | DNA origami-based binary deoxyribozyme structure and its preparation method and application |
| abstract | The invention provides a DNA origami-based binary deoxyribozyme structure and a preparation method and application thereof. The preparation method comprises: forming a DNA origami structure by annealing; The enzyme strand b is embedded in the DNA origami structure; the helper chain is added to promote the successful assembly of the dimeric deoxyribozyme on the DNA origami structure; the double-labeled substrate strand is added to verify the efficient assembly of the dimeric deoxyribozyme by the fluorescent signal Due to the effect of the confinement space of the DNA origami structure, the present invention improves the collision probability between the helper chain and the streptases a and b by embedding the two-part deoxyribozyme on the DNA origami structure, and also reduces the energy of hybridization. The potential barrier allows the two-part DNAzyme to be assembled on the DNA origami structure; because the double-labeled reporter substrate can be used universally, it not only effectively reduces the DNA sequence requirements for streptases a and b, but also reduces the complexity of multiplex analysis. synthetic cost. |
| priorityDate | 2021-02-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 26.