| abstract |
The invention discloses an in vitro culture method of γδT cells, which belongs to the technical field of cell culture. The invention discloses a method for culturing γδT cells in vitro, comprising the following steps: obtaining peripheral blood mononuclear cells; adjusting the cell density by using RPMI1640 medium containing fetal bovine serum, adding zoledronic acid and interleukin-2, and culturing; During the period, the fresh complete medium was replaced, and the cells were expanded and cultured; the cell purity was detected by flow cytometry; the mature γδT cells were harvested. A method for culturing γδT cells in vitro disclosed by the invention adopts the density gradient centrifugation method to obtain peripheral blood mononuclear cells, which is simple and easy to implement; the in vitro induction and culture system of γδT cells is clear and clear, and the expansion multiple is high; the γδT cells prepared by the invention The immune effect is good, and the safety risk is low. |