| abstract |
The invention belongs to the field of tumor therapy and molecular immunology, and relates to an anti-VEGFA-anti-PD-1 bispecific antibody and use thereof. Specifically, the anti-VEGFA-anti-PD-1 bispecific antibody includes: a first protein functional domain targeting PD-1 and a second protein functional domain targeting VEGFA, wherein, according to the EU numbering system , the heavy chain constant region of the immunoglobulin contained in the bispecific antibody is mutated at two sites, 234 and 235, and after the mutation, the bispecific antibody interacts with FcγRI, FcγRIIa, FcγRIIIa and/or C1q The affinity constant decreased compared to that before mutation. The bispecific antibody of the present invention can specifically bind to VEGFA and PD-1, specifically relieve the immunosuppression of VEGFA and PD-1 on the body, and at the same time inhibit tumor-induced angiogenesis, and has a good application prospect. |