http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112813189-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-158 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6895 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6895 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 |
filingDate | 2021-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2023-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2023-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-112813189-B |
titleOfInvention | A method for rapid identification of transgenic maize lines using quadruple real-time fluorescent PCR |
abstract | The invention discloses a method for rapidly identifying transgenic maize strains by using quadruple real-time fluorescent PCR, which comprises the following steps: selecting three transgenic maize strains MIR162, Mon89034, and Bt11 and the endogenous maize gene zSSIIb as internal reference genes; designing four Primers and probes required for real-time fluorescent PCR amplification; extraction of genomic DNA from the corn sample to be tested; quadruple real-time fluorescent PCR amplification system setup; determination of the corn transgenic line to be tested or whether it contains a certain line of transformant components. The invention can realize simultaneous detection of specific gene sequences of three corn strains MIR162, Bt11 and Mon89034 and one endogenous gene of corn in one reaction tube, reducing reagent cost and shortening detection time. |
priorityDate | 2021-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 376.