http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112813120-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cc4e13141d402ceb885f368dfa042348 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate | 2020-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ddf452fbd59590298416f7e47eee5c1c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ae2c8481d73793914eaf933e0974c525 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1cea4df70aed67b177a6a12bedaed6f8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b7353bf0206dc1c19cb03217a4a418ac http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b4d8f92a44f895e7486608554b5d524c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0ea5d8eca560e95edecd79dd2bda6578 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_335ccc94a85809b3389ecf68c8cf8caf |
| publicationDate | 2021-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112813120-A |
| titleOfInvention | A method for the efficient synthesis of 5'-labeled RNA using DNA polymerase mutant strains |
| abstract | The invention belongs to the field of biotechnology, and discloses a method for efficiently synthesizing 5'-labeled RNA by using a DNA polymerase mutant strain. The method includes: uniformly mixing the primers of the single-stranded DNA containing the target sequence, the polymerase buffer solution, and the DNA oligonucleotide chain with the modified 5' end, heating up to remove the spatial structure, and then cooling down and annealing to obtain the mixed system 1; Add ribonucleotides and SFM4-3 polymerase to the mixing system 1, mix evenly to obtain a transcription system, incubate, purify and isolate RNA, and obtain 5'-labeled RNA of the target sequence. In the method provided by the invention, the thermostable DNA polymerase obtained by directional transformation can directly label the 5' end of all RNA products with fluorescein or biotin when synthesizing RNA, and while realizing the labeling, the RNA molecules are still kept intact. activity and function. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113604524-A |
| priorityDate | 2020-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 88.