| abstract |
The invention discloses an anti-glycosyltransferase A subunit nanobody and application thereof. The variable region of the nanobody has three complementarity determining regions CDR-1, CDR-2 and CDR-3, and CDR-1 is represented by SEQ ID NO: 1. The amino acid sequence shown in ID NO.1 is formed, CDR-2 is formed by the amino acid sequence shown in SEQ ID NO.2, and CDR-3 is formed by the amino acid sequence shown in SEQ ID NO.3. The anti-glycosyltransferase A subunit nanobody provided by the present invention has the activity of specifically binding to the glycosyltransferase A subunit, neutralizes the activity of the glycosyltransferase A, and can be used to detect Clostridium difficile, and also It can be used to prepare a drug for the treatment of Clostridium difficile; the nanobody also has the advantages of small molecular weight, high affinity, stable structure and performance, etc.; it can withstand the acidic environment of the stomach and is not easily degraded by pepsin. |