| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_db9efde660b78f98b102046e09d07ab7 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-70532
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2458-10 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-582
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-57415
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-57492 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-574
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 |
| filingDate |
2021-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7592b1f2ae1c67915c59c7267473ab2a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9007c90744467900f44b6fc7b3c437f2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_226427498ec04e4242b0e96e274de9a9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8405ba205d776ab3f4988919cc8a0c27 |
| publicationDate |
2021-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-112798796-A |
| titleOfInvention |
DNA-PAINT-based detection method and sequence of PD-L1 protein on cell membrane |
| abstract |
The invention discloses a method and a sequence for detecting PD-L1 protein on a cell membrane based on DNA-PAINT, which utilize a designed DNA anchor chain and a DNA imaging chain to carry out DNA-PAINT imaging by cell fixation, antibody coupling and incubation and adopting a total internal reflection illumination mode for detecting PD-L1 protein on the surface of a breast cancer cell. By utilizing the advantages of no influence of fluorescence quenching, high spatial resolution and the like of a DNA-PAINT super-resolution technology, the distribution of PD-L1 protein on a membrane on MB-231 cells and MCF-7 cells is detected, and certain help is provided for immunotherapy of breast cancer. |
| priorityDate |
2021-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |