abstract |
The invention relates to the technical field of animal disease detection, in particular to a recombinase-mediated isothermal amplification method that can rapidly detect porcine pseudorabies virus, specifically including primer pairs, probes and kits used in the detection process. The RAA primer pair and probe for detecting porcine pseudorabies virus and the corresponding kit according to the invention can realize the rapid detection of porcine pseudorabies virus, and have the characteristics of simplicity, rapidity, sensitive response and good specificity, and the amplified product The results can be judged visually by the portable blue light meter, which can truly realize portable rapid nucleic acid detection. |