| abstract |
The invention belongs to the technical field of biological pathogen detection, and in particular relates to a specific PCR amplification primer pair for detecting H. albicans, a detection method and application. The detection method first extracts the DNA of honeybee larvae, and then uses primer pairs WxlF2: 5'-ACTGATCCAACAGATCCAAC-3', WxlR2: 5'-TCCCTTAAAGTCAGACAAGC-3'; or WxlF3: 5'-AACAGATCCAACTGGTCAAA-3', WxlR3: 5'-CTTAAAGTCAGACAAGCCGA ‑3' is subjected to PCR amplification, and finally, electrophoresis is carried out to observe the amplification situation to determine whether or not to carry H. albicans. The detection method does not need to separate a single colony of H. albicans, is simple and easy to implement, and the PCR amplification time can be controlled within 1 hour, which can meet the needs of rapid detection of European larval rancidity. |