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filingDate 2021-01-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_749bbe00b3b69d406f3f6a42122e56d9
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publicationDate 2021-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-112795649-A
titleOfInvention A probe set for detecting the amplification level of HER2 gene and its application
abstract The present invention relates to the technical field of gene detection, in particular to a probe set for detecting the amplification level of HER2 gene and its application. The probe set is prepared from the nucleotide sequence shown in Table 1 of the specification. In the non-repetitive region of the HER2 gene, a variety of oligonucleotide libraries of different lengths are used to increase the probe resolution on the basis of using as few probes as possible; the PCR incorporation method is also used in the preparation process. , to ensure that the product contains more fluorescent groups, the above probe set is used to detect the amplification level of the HER2 gene, and the FISH hybridization of paraffin tissue samples can be completed within 30 minutes, with high sensitivity, specificity and hybridization. The background is clean and the signal-to-noise ratio is low.
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