| abstract |
The invention relates to a preparation method of improved heat-resistant bacillus cereus collagenase, wherein the original bacillus cereus collagenase sequence is improved by introducing X to Y mutations, the bacillus cereus collagenase mutant sequences are SEQ ID NO1, SEQ ID NO2, SEQ ID NO3, SEQ ID NO4, SEQ ID NO5, SEQ ID NO6 and SEQ ID NO7, and the enzyme with the mutant sequence SEQ ID NO7 is preferably obtained, so that the enzyme has better application prospect and obviously improved heat stability while the enzyme activity is kept. |