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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-415
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filingDate 2019-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2022-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2022-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-112779280-B
titleOfInvention A seed-specific interference vector comprising pOsOle18 promoter and its application
abstract The invention belongs to the field of biotechnology, in particular to a seed-specific interference vector comprising a pOsOle18 promoter and its application. Specifically, a seed-specific interference vector is provided, and the vector is a pOsOle18-RNAi vector, and its DNA sequence is shown in SEQ ID NO 9. The pOsOle18-RNAi vector can specifically interfere with the expression of the target gene in rice seeds without affecting the normal expression of the target gene in other tissues and organs, thereby not only effectively studying the direct regulation effect of the target gene on seed development It can also achieve the purpose of improving rice grain shape, yield and rice quality, and provide an effective technical means and solution for molecular precision design and breeding of rice yield and rice quality. At the same time, an OsMADS1 seed-specific interference vector pOsOle18::OsMADS1-RNAi based on the pOsOle18-RNAi vector is also provided, the DNA sequence of which is shown in SEQ ID NO 10; the use of the vector can improve the protein content in the rice, and increase the protein content of the rice. nutritional value. The invention has important scientific research significance and application value.
priorityDate 2019-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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