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filingDate 2021-02-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2021-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-112779248-A
titleOfInvention An integrated nucleic acid detection cartridge for Chlamydia trachomatis
abstract The invention discloses an integrated nucleic acid detection cartridge for Chlamydia trachomatis, comprising a box body with a box cover on the top, and a lysis chamber, a first cleaning chamber and a second cleaning chamber are sequentially arranged in the box body from top to bottom, wherein the lysis chambers communicate with each other. To the box cover, the second cleaning chamber is connected to the reaction chamber provided at the bottom of the outer side of the box body; the two connected sections of the cracking chamber, the first cleaning chamber, the second cleaning chamber and the reaction chamber are respectively provided with plunger holes whose direction is perpendicular to The plunger in the connecting direction, the plunger hole of the plunger is provided with a hydrophobic liquid sealing material; the reaction chamber is provided with an amplification reaction liquid, the reaction chamber is provided with a fusible material layer, and the soluble material layer is provided with Primer-probe mixture containing the Aomp gene of Chlamydia trachomatis. The invention can rapidly and integrally carry out PCR amplification and detection of Chlamydia trachomatis at relatively low cost, and solves the problems of poor repeatability of the current technology, time-consuming detection method, complicated operation and the like.
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