| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5456846495c5fa8a02f5eaae9faae290
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f364d68ebbab6ebcd83c6ded4a2044cc |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02E50-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-21 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A23K20-189
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2482
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A23L29-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01008
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-00 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A23K20-189
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-56
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A23L29-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-42 |
| filingDate |
2021-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5aa107bfc186be4fc6470c007bc72b70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3f8159f3d9167c2ac7b391bbc9a26963
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ffb28a3a37c0abe1545a3df61609546a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2fcf630754526034aac11947125cc63c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b69e28788b3dd460199fa8377918747d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5b21a44a919a8b6f08c5051f87c1e325 |
| publicationDate |
2021-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-112779241-A |
| titleOfInvention |
A metagenomic-derived xylanase and its encoding gene |
| abstract |
The invention discloses a metagenome-derived xylanase encoding gene and a method for its high-efficiency expression and product separation and purification. The inventors cloned a xylanase gene from the papermaking wastewater metagenomic library, and the product xylanase expressed in Escherichia coli can hydrolyze glycosidic bonds in an endo-cut manner. After one-step separation and purification of the expressed crude enzyme by Ni magnetic bead method, the specific activity was 438.4 U/mg at the optimum pH 7.0 and 60 ℃. The enzymatic properties study found that the enzyme had good activity between pH 5.0 and 8.0, and the enzyme activity remained above 75% after being incubated at 65°C for 60 minutes. The enzyme has a wide range of pH adaptation and good thermal stability, and can well meet the needs of industrial production. |
| priorityDate |
2021-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |