| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_729ac72bc03053dfb64ff7dc881c541a |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-60
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y105-01003
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-78
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y305-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-003 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-78
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 |
| filingDate |
2020-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6b88d56acca72cab6da52dfa7c37a254
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_decdc2abb3271d782a3c5484c37644fa
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4c646eba161b250dadbe03c9aa6293a5 |
| publicationDate |
2021-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-112778425-A |
| titleOfInvention |
A kind of preparation method of RNA gene editing system that reduces off-target effect |
| abstract |
The invention relates to the technical field of gene editing, in particular to a preparation method of an RNA gene editing system that reduces off-target effects. A fusion protein provided by the present invention includes the amino acid sequence of Cas13b protein, the amino acid sequence of ADAR2 protein and the amino acid sequence with dihydrofolate reductase activity from the N-terminus to the C-terminus in turn; the above-mentioned fusion protein is applied to RNA gene editing systems such as In the RESCUE system, by fusing the amino acid sequence with dihydrofolate reductase activity at the carbon end, the amino acid sequence with dihydrofolate reductase activity can combine with TMP to regulate the expression of Cas13b protein by dRanCas13b and the expression of ADAR2 protein by hADAR2 without affecting the editing efficiency. On the premise, the number of off-target SNPs in the RNA editing process can be significantly reduced, thereby improving the application safety of the RNA single base editing system. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115369124-A |
| priorityDate |
2020-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |