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filingDate 2021-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2022-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2022-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-112760334-B
titleOfInvention Gene regulating sugar content in tomato fruit and its application
abstract The invention relates to the fields of biotechnology and genetic breeding, in particular to a gene for regulating sugar content in tomato fruit and its application. The gene for regulating tomato fruit sugar content provided by the present invention is SlCIN2, and its nucleotide sequence is shown in SEQ ID NO.1. The invention first extracts tomato RNA, reversely transcribes cDNA, designs amplification primers, uses cDNA as a template to amplify SlCIN2 gene; uses gateway technology to construct SlCIN2 gene into pB7GWIWG2 vector, screen correct positive clones, and obtain positive clones Transform into LBA4404 Agrobacterium competent to obtain recombinant Agrobacterium, and transform the recombinant Agrobacterium into tomato to obtain tomato plants with increased fruit sugar content.
priorityDate 2021-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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