http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112760289-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-724 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-998 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-405 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2513-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-392 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-115 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-15 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0693 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0631 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-09 |
| filingDate | 2021-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112760289-B |
| titleOfInvention | Special culture medium for breast cancer organoid and 3D culture method |
| abstract | The invention provides a special culture medium for breast cancer organoid and a 3D culture method, wherein the culture medium comprises a basic culture medium and a specific additive factor; the specific addition factor comprises GSK 429286A; doramapimod; a83-01; EGF;beta-estradiol; cholera Toxin; FGF 2; HEPES (high efficiency particulate matter); b27; GlutaMAX; an ITS; gentamicin; neuredulin-1; BSA. The culture method comprises the following steps: pretreating the separated breast cancer tissue, digesting and filtering to obtain cell sediment; cracking cell precipitate, mixing with matrigel gel, inoculating, coagulating, adding the culture medium, and culturing at 37 deg.C under 5% CO 2 Culturing at the concentration for 7-14 days. The culture medium can realize the rapid growth of breast cancer organoids, can be stably cultured for a long time, and can well keep the heterogeneity of tumor tissues of patients in vitro, and the formed tumor organoids have regular spherical cells and uniform sizes. |
| priorityDate | 2021-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 68.