http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112725331-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2021-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112725331-B |
| titleOfInvention | A method for constructing a high-throughput mutant library |
| abstract | The invention belongs to the technical field of bioengineering, and in particular relates to a general construction method of a high-throughput mutant library. The mutant library construction technology provided by the present invention uses the double codon coding technology to design batch-synthesized long-fragment primers as mutant templates, uses high-efficiency cloning technology to connect the mutant fragments into a receiving vector, and then adds a tag sequence to the public area of the vector to make . The mutant library construction method provided by the present invention has the advantages of simple technology, high reliability and low comprehensive cost, and can be used for the directed evolution of important functional proteins such as enzymes and antibodies, and can also be used for cancer, genetic diseases, metabolic diseases and drugs. The biological significance of mutants of genes in the genome and other fields is studied. |
| priorityDate | 2021-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 140.