http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112725301-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07007 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1252 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 |
| filingDate | 2021-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112725301-B |
| titleOfInvention | Taq DNA polymerase mutants and their applications |
| abstract | The invention discloses a Taq DNA polymerase mutant and its application. The present invention provides a single-site mutant of Taq DNA polymerase with improved thermostability, which is obtained by performing G79E, G80A, D177E, A180V, E189P, S357A, A472E or G504K on the amino acid sequence shown in SEQ ID No. 1. mutants obtained by single-site mutation in . On the basis of single-site mutation, the present invention provides a Taq DNA polymerase multi-site mutant with further improved thermal stability. The qPCR thermal stability test shows that the single-site or multi-site mutants of the Taq DNA polymerase provided by the present invention have significantly improved enzyme activity compared with the wild-type enzyme, and the thermal stability is obviously better than that of the wild-type Taq DNA polymerase. The present invention also provides the use of the mutant as Taq DNA polymerase in gene amplification. |
| priorityDate | 2021-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 324.