http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112725300-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d9de52bba13e16028dffa8775e3f3f28 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07007 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1252 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2021-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aaeb2d57e1e7aa1d250e272dfca8f5ac http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a481f4535bcb96ac8f404c4db8fe013a |
| publicationDate | 2021-04-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112725300-A |
| titleOfInvention | A kind of DNA polymerase γ and its encoding gene, preparation method and application |
| abstract | The present invention provides a DNA polymerase γ and its encoding gene, preparation method and application. The DNA polymerase γ is derived from bacteriophage. The DNA polymerase γ of the present invention has DNA polymerase and exonuclease activities, and also has activity in the presence of calcium ions, and can be used to extend DNA and prepare DNA, and the activity in the presence of calcium ions can be used to detect the presence of calcium ions. DNA polymerase gamma was cloned into the pET‑sumo expression vector, and the sumo protease tag sequence was used to improve the solubility of DNA polymerase gamma. The recombinant expression vector was induced to express in E. coli, purified by immobilized nickel ion affinity, and finally protease excised the sumo tag to prepare DNA polymerase γ. |
| priorityDate | 2021-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 500.