| abstract |
The invention provides an NK trophoblast cell and application thereof, wherein the NK trophoblast cell expresses a cytokine composition, and the cytokine composition comprises CD86, CD19, IL-21, CD137L and CD 64; the CD86 comprises an amino acid sequence shown in SEQ ID No. 1. The invention also provides a recombinant vector, a recombinant lentivirus, a preparation method of the NK trophoblast cell and an NK cell culture medium. The recombinant vector is constructed and packaged to obtain the recombinant lentivirus, and then the recombinant lentivirus is introduced into the genome of a host cell, so that the prepared NK trophoblast cell can continuously secrete related proteins for stimulating the proliferation and differentiation of the NK cell, the high efficiency and low-cost amplification of the NK cell are realized, and the preparation method is simple and efficient and has wide application prospect. |