| abstract |
The invention belongs to the technical field of cell culture, in particular, the invention relates to a serum-free medium additive and its preparation method and application. The additive for serum-free medium of the present invention can replace animal source components such as serum and platelet lysate through the synergistic ratio of the three components. The additive has clear ingredients, good repeatability between different batches, and high clinical safety. , to meet the needs of in vitro culture of mesenchymal stem cells from different sources such as fat, bone marrow, umbilical cord, umbilical cord blood, and placenta. Furthermore, the additive of the serum-free medium of the present invention is freeze-dried powder, which can be stored for a long time, has high stability, and the activity of each component is not easily lost, and is suitable for long-term storage and transportation. |