http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112714796-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_eb141b396e502bfce986c491d722e401 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6865 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-93 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-12 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6865 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6886 |
| filingDate | 2019-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8ff3eb06b5532a5cc35fc80ec4154ae5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3937ccc49851a630346073a2dde27a72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bf00905adefdb49a5ea40fd0ea455e1b |
| publicationDate | 2021-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112714796-A |
| titleOfInvention | Method for amplifying bisulfite-treated DNA |
| abstract | The methods, compositions and kits of the present disclosure provide novel approaches for genome-wide, ie, unbiased DNA analysis, that can be performed on limited amounts of DNA. It can be used to analyze DNA to determine its modification status. Aspects of the present disclosure relate to methods for amplifying bisulfite-treated deoxyribonucleic acid (DNA) molecules, the methods comprising: (a) ligating an adaptor to the DNA molecule, wherein the adaptor comprises an RNA polymerase a promoter comprising a bisulfite-protected cytosine; (b) treating the ligated DNA molecule with bisulfite; (c) hybridizing the bisulfite-treated DNA molecule to a primer; (d) ) extending the hybridized primer to generate double-stranded DNA; and (e) transcribing the double-stranded DNA in vitro to generate RNA. |
| priorityDate | 2018-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 289.