http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112708610-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0625 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6489 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6427 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-21004 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-76 |
| filingDate | 2021-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112708610-B |
| titleOfInvention | Mixed enzyme for skin tissue dissociation, preparation method thereof, dissociation kit and dissociation method |
| abstract | The invention discloses a mixed enzyme for skin tissue dissociation, which consists of collagenase II, trypsin and deoxyribonuclease I; the concentration of the collagenase II is 2-4 mg/mL; the concentration of the trypsin is 1-3 mg/mL; the concentration of the DNase I is 0.01-0.05 mg/mL. In addition, the invention also discloses a dissociation method for skin tissues, which is characterized in that the skin tissues are pretreated by a specific buffer solution, and the specific mixed enzyme is adopted for tissue dissociation, so that the skin tissue single cell suspension with more total number of single cells, good activity, less fragments and low agglomeration rate can be efficiently, quickly and stably obtained. |
| priorityDate | 2021-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 73.